6VH4

Wild type EGFR in complex with LN2380

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978801
Spacegroup name	I 2 3
Unit cell lengths	146.534, 146.534, 146.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.160 - 2.800
R-factor	0.1969
Rwork	0.195
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d8e
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.170	39.170	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.121	0.050	0.759
Rmeas	0.134	0.055	0.850
Rpim	0.056	0.022	0.373
Total number of observations	65862	761	4626
Number of reflections	12526	139	930
<I/σ(I)>	9.3	18.4	2.1
Completeness [%]	96.7	86.7	97.4
Redundancy	5.3	5.5	5
CC(1/2)	0.994	0.997	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.4 M Sodium Citrate, 0.1 M MES