6VGJ
N-terminal variant of CXCL13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-04 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.761, 41.567, 111.769 |
| Unit cell angles | 90.00, 102.12, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.520 |
| Rwork | 0.249 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZAI |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_3946) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 6.830 | 2.520 |
| Rpim | 0.058 | 0.032 | 0.919 |
| Number of reflections | 21429 | 1161 | 924 |
| <I/σ(I)> | 12.39 | ||
| Completeness [%] | 98.6 | 99.7 | 86.5 |
| Redundancy | 4.8 | 4.8 | 2.6 |
| CC(1/2) | 0.990 | 0.993 | 0.356 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 0.1 M Citric acid pH 3.5, 14% w/v PEG 1000 |
