6VG4

Human protocadherin 10 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	84.087, 84.087, 543.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.298
R-factor	0.2297
Rwork	0.227
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vfv 6vfq
RMSD bond length	0.010
RMSD bond angle	0.674
Data reduction software	XDS (1.10.1_2155)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.480
High resolution limit [Å]	3.298	3.298
Rmerge	0.139	2.475
Rmeas	0.145	2.570
Rpim	0.040	0.687
Number of reflections	19779	4386
<I/σ(I)>	10.7	1.6
Completeness [%]	99.7	99.5
Redundancy	12.9	13.7
CC(1/2)	1.000	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	0.22M CaCl2, 0.1M Na Acetate pH 5.0 with 30% (v/v) glycerol as cryoprotectant and glutaraldehyde (0.5% v/v) added to the reservoir after crystal formation to aid crystal stability by cross-linking