6VG1

xenopus protocadherin 8.1 EC1-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	146.645, 42.393, 167.471
Unit cell angles	90.00, 110.60, 90.00

Refinement procedure

Resolution	38.270 - 2.000
R-factor	0.2544
Rwork	0.253
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vfq 6vfv
RMSD bond length	0.006
RMSD bond angle	0.814
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.130	5.280
Number of reflections	59475	6132
<I/σ(I)>	5.8
Completeness [%]	97.5
Redundancy	4.4
CC(1/2)	1.000	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.135M nitrate-phosphate-sulfate mix (0.045M sodium phosphate dibasic dihydrate, 0.045M Ammonium sulfate, 0.045 M sodium nitrate; Molecular Dimensions), 0.1M MES/imidazole buffer pH 6.5 with no further cryoprotection