6VFV

Crystal structure of human protocadherin 8 EC5-EC6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	41.658, 29.498, 92.442
Unit cell angles	90.00, 98.46, 90.00

Refinement procedure

Resolution	19.900 - 2.900
R-factor	0.227
Rwork	0.225
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5szr
RMSD bond length	0.005
RMSD bond angle	0.772
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.070
High resolution limit [Å]	2.900	2.900
Rmerge	0.130	0.570
Rmeas	0.160	0.720
Rpim	0.080	0.440
Number of reflections	5168	803
<I/σ(I)>	8.2	1.7
Completeness [%]	99.6	99.1
Redundancy	3.3	2.3
CC(1/2)	0.990	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	10% PEG 20 000 (w/v), 20% (v/v) PEG 550 monomethylether, 0.03M diethyleneglycol, 0.03M triethyleneglycol, 0.03M tetraethyleneglycol, 0.03M pentaethyleneglycol, 0.1M MES/imidazole buffer pH 6.5