6VFT

Crystal structure of human delta protocadherin 17 EC1-EC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	214.550, 105.838, 101.403
Unit cell angles	90.00, 101.04, 90.00

Refinement procedure

Resolution	19.940 - 3.710
R-factor	0.2552
Rwork	0.253
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vfq
RMSD bond length	0.008
RMSD bond angle	0.821
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.000
High resolution limit [Å]	3.700	3.700
Rmerge	0.211	0.735
Rmeas	0.248	0.866
Rpim	0.128	0.451
Number of reflections	22957	4468
<I/σ(I)>	5.4	1.6
Completeness [%]	96.7	92.1
Redundancy	3.5	3.4
CC(1/2)	1.000	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	13%(w/v) PEG 4000 0.3M NaCl 0.1M MES pH 6.5 30% PEG 400 (v/v) added as cryoprotectant