6VFR

Crystal structure of human protocadherin 18 EC1-EC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	126.959, 176.942, 71.872
Unit cell angles	90.00, 101.42, 90.00

Refinement procedure

Resolution	19.900 - 2.790
R-factor	0.2376
Rwork	0.236
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vfq
RMSD bond length	0.014
RMSD bond angle	0.755
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.920
High resolution limit [Å]	2.790	2.790
Rmerge	0.083	1.240
Rmeas	0.094	1.410
Rpim	0.042	0.640
Number of reflections	38201	4506
<I/σ(I)>	10.2	1.4
Completeness [%]	97.2	91.4
Redundancy	4.8	4.6
CC(1/2)	1.000	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	13% (w/v) PEG 20 000, 0.1M MES pH 6.5, with 30% (v/v) ethylene glycol added as cryoprotectant