6VF6

DNA Polymerase Mu, 8-oxorGTP:At Product State Ternary Complex, 50 mM Mn2+ (960 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.263, 62.333, 118.847
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.435 - 1.690
R-factor	0.1667
Rwork	0.166
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.720
High resolution limit [Å]	1.690	4.590	1.690
Rmerge	0.081	0.052	0.993
Rmeas	0.088	0.056	1.092
Rpim	0.034	0.022	0.444
Total number of observations	331237
Number of reflections	50793	2746	2474
<I/σ(I)>	10.1
Completeness [%]	99.8	99.3	99.8
Redundancy	6.5	6.4	5.9
CC(1/2)		0.995	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000