6VF3

DNA Polymerase Mu, 8-oxorGTP:At Ground State Ternary Complex, 50 mM Mn2+ (15 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.198, 62.385, 118.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.389 - 1.521
R-factor	0.1635
Rwork	0.163
R-free	0.17990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.520	4.130	1.520
Rmerge	0.055	0.042	0.826
Rmeas	0.060	0.047	0.897
Rpim	0.024	0.020	0.345
Total number of observations	431742
Number of reflections	68645	3632	3360
<I/σ(I)>	11.9
Completeness [%]	99.1	97.1	98.9
Redundancy	6.3	5.8	6.5
CC(1/2)		0.995	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000