6VF2

DNA Polymerase Mu, 8-oxorGTP:At Product State Ternary Complex, 50 mM Mg2+ (960 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.278, 62.297, 118.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.977 - 1.600
R-factor	0.1693
Rwork	0.168
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.072	0.055	0.917
Rmeas	0.077	0.060	0.990
Rpim	0.029	0.023	0.367
Total number of observations	422680
Number of reflections	59671	3210	2951
<I/σ(I)>	10.6
Completeness [%]	100.0	99.6	99.9
Redundancy	7.1	6.7	7
CC(1/2)		0.994	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000