6VF1

DNA Polymerase Mu, 8-oxorGTP:At Product State Ternary Complex, 50 mM Mg2+ (120 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.044, 68.415, 111.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.023 - 1.680
R-factor	0.1638
Rwork	0.163
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.710
High resolution limit [Å]	1.680	4.560	1.680
Rmerge	0.075	0.032	0.996
Rmeas	0.084	0.036	1.127
Rpim	0.038	0.016	0.518
Total number of observations	242380
Number of reflections	52575	2867	2616
<I/σ(I)>	14.6
Completeness [%]	99.3	99.8	99.5
Redundancy	4.6	4.6	4.3
CC(1/2)		0.998	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000