6VD8

Metal-bound C-terminal domain of CzcD transporter from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.2652
Spacegroup name	P 21 21 21
Unit cell lengths	36.916, 58.247, 72.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.290 - 2.300
R-factor	0.24102
Rwork	0.238
R-free	0.27137
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	1.154
Data reduction software	XDS (5.8.0238)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (7.0.073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.290	2.370
High resolution limit [Å]	2.290	2.290
Number of reflections	7349	653
<I/σ(I)>	10.2
Completeness [%]	99.3
Redundancy	25.6
CC(1/2)	0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium acetate, 0.1 M Bis-Tris pH 7.2, 30% (w/v) PEG 3350, 1.0 mM ZnCl2