6VD1
Crystal structure of Arabidopsis thaliana S-adenosylmethionine Synthase 2 (AtMAT2) in complex with S-adenosylmethionine and PPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.205, 101.484, 84.334 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 47.090 - 1.320 |
| R-factor | 0.1418 |
| Rwork | 0.142 |
| R-free | 0.17320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vcz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.810 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.090 | 1.400 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.056 | 0.585 |
| Number of reflections | 232535 | 37677 |
| <I/σ(I)> | 12.45 | 2.03 |
| Completeness [%] | 97.8 | 98.2 |
| Redundancy | 3.35 | 3.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.12 M Alcohols (1,6-hexanediol; 1-butanol; 1,2-propanediol; 2-propanol; 1,4-butanediol; 1,3-propanediol), 0.1 M HEPES and MOPS buffer at pH 7.5, 20% mmPEG500, 10% PEG 20000 |
