6VCY
Crystal structure of Arabidopsis thaliana S-adenosylmethionine Synthase 1 (AtMAT1) in complex with 5'-methylthioadenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 58.280, 69.156, 210.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.250 - 1.820 |
| R-factor | 0.1603 |
| Rwork | 0.159 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vcx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.922 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.250 | 1.930 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.071 | 0.774 |
| Number of reflections | 37699 | 5980 |
| <I/σ(I)> | 12.53 | 1.84 |
| Completeness [%] | 97.8 | 97.3 |
| Redundancy | 3.7 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 1.8 M (NH4)2SO4, 0.08 M Bis-Tris |
