6VCK
Crystal structure of E.coli RppH-DapF in complex with GDP, Mg2+ and F-
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 161.840, 193.601, 51.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.930 - 2.690 |
R-factor | 0.2301 |
Rwork | 0.226 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d1v |
RMSD bond length | 0.005 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.930 | 2.800 |
High resolution limit [Å] | 2.690 | 2.700 |
Number of reflections | 22647 | 2203 |
<I/σ(I)> | 24.56 | |
Completeness [%] | 99.4 | |
Redundancy | 6.6 | |
CC(1/2) | 0.995 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 291.15 | 30% (v/v) PEG400, 0.1 M CHES, pH 9.2 |