6VC8
Crystal structure of wild-type KRAS4b(1-169) in complex with GMPPNP and Mg ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.210, 82.330, 88.140 |
| Unit cell angles | 90.00, 112.92, 90.00 |
Refinement procedure
| Resolution | 37.910 - 2.500 |
| R-factor | 0.2155 |
| Rwork | 0.212 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gft |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.910 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.053 | 0.565 |
| Number of reflections | 15480 | 1571 |
| <I/σ(I)> | 15.5 | 2.02 |
| Completeness [%] | 97.3 | 97.3 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 32% PEG 4000, 800 mM LiCl, 100 mM Magnesium Chloride and 100 mM Tris pH 8.5 |
