6VBP
Crystal structure of anti-HIV-1 antibody DH815 bound to gp120 V2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-02 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.903, 158.531, 92.646 |
Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
Resolution | 45.152 - 2.298 |
R-factor | 0.1815 |
Rwork | 0.180 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmt |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.14_3228) |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.298 | 2.298 |
Rpim | 0.075 | 0.483 |
Number of reflections | 80681 | 6592 |
<I/σ(I)> | 9.31 | |
Completeness [%] | 95.9 | 77.8 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 292 | 0.2M sodium chloride, 20% PEG 8000 and 0.1M sodium citrate pH 4.2. |