6VAG

Crystal structure of the oligomerization domain of phosphoprotein from parainfluenza virus 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	29.893, 36.048, 140.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.340 - 1.400
R-factor	0.1559
Rwork	0.155
R-free	0.17970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ideal helices
RMSD bond length	0.005
RMSD bond angle	0.693
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmeas	0.065
Rpim	0.025
Number of reflections	28002	1239
<I/σ(I)>	38.12	2.08
Completeness [%]	90.8
Redundancy	6.5	7.1
CC(1/2)		0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1 M MIB buffer (malonic acid, imidazone, boric acid), pH 6.0 and 25% w/v PEG 1500