6V8U

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a modified Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	C 2 2 2
Unit cell lengths	44.142, 183.624, 97.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.919 - 2.103
R-factor	0.1753
Rwork	0.172
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.920	2.178
High resolution limit [Å]	2.103	2.103
Rmerge	0.107	0.777
Rmeas	0.112
Number of reflections	23073	1986
<I/σ(I)>	17.6	2.83
Completeness [%]	97.6	86.23
Redundancy	11.1	6.6
CC(1/2)	0.996	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.02 M Calcium Chloride, 0.1 M Sodium Acetate, 30 % 2-methyl-2,4-pentanediol