6V8M
Crystal structure of Ara h 8.0201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.668, 42.012, 81.936 |
| Unit cell angles | 90.00, 124.25, 90.00 |
Refinement procedure
| Resolution | 30.760 - 1.750 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v8j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.054 | 0.566 |
| Rmeas | 0.062 | 0.655 |
| Rpim | 0.031 | 0.324 |
| Number of reflections | 33202 | 1656 |
| <I/σ(I)> | 34 | 2.4 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.1M HEPES, 2.0M Ammonium Sulfate, pH 8.0 |
