6V81

The crystal structure of the outer-membrane transporter YncD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 2 21
Unit cell lengths	87.940, 98.640, 124.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.970 - 2.957
R-factor	0.2789
Rwork	0.277
R-free	0.31420
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.320	3.140
High resolution limit [Å]	2.957	2.960
Rmerge	0.160	0.735
Rpim	0.072	0.363
Number of reflections	17684	448
<I/σ(I)>	8.7	0.6
Completeness [%]	75.2	11.7
Redundancy	6.7	5.8
CC(1/2)	0.999	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	17% PEG3000, 0.2 M CaAcetate, 0.1 M Tris