6V7T
Crystal structure of CTX-M-14 E166A/D240G beta-lactamase in complex with ceftazidime
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999974 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.110, 107.330, 47.750 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 47.040 - 1.340 |
| R-factor | 0.1439 |
| Rwork | 0.143 |
| R-free | 0.16160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.956 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.047 | 0.118 |
| Rmeas | 0.055 | 0.137 |
| Rpim | 0.028 | 0.068 |
| Number of reflections | 99523 | 9924 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.4 | 99.26 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M Na chloride, 0.1M Tris pH 8, 20% PEG 6000 |
