6V7P
Crystal structure of SUMO1 in complex with PIAS-SIM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.10 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.350, 38.467, 62.745 |
Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
Resolution | 30.604 - 1.395 |
R-factor | 0.1543 |
Rwork | 0.152 |
R-free | 0.18380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uyo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.604 | 1.445 |
High resolution limit [Å] | 1.395 | 1.395 |
Number of reflections | 30629 | 2526 |
<I/σ(I)> | 15.38 | |
Completeness [%] | 95.3 | |
Redundancy | 3.1 | |
CC(1/2) | 0.998 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium cacodylate pH 6.5, 19 to 31 % (w/v) PEG3350 and 10 mM calcium chloride. |