6V5F
L-asparaginase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2017-08-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.4587 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.917, 125.435, 68.816 |
Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
Resolution | 33.370 - 2.100 |
R-factor | 0.2325 |
Rwork | 0.230 |
R-free | 0.29760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eca |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.199 | 3.401 |
Number of reflections | 105403 | 16633 |
<I/σ(I)> | 5.38 | 0.33 |
Completeness [%] | 97.7 | 95.1 |
Redundancy | 3.15 | 2.413 |
CC(1/2) | 0.994 | 0.214 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 0.1 M succinic acid pH 7.0, 15% (w/v) polyethylene glycol 3,350 |