6V56
The crystal structure of the 2009 H1N1 PA endonuclease wild type in complex with SJ000985494
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 89.546, 89.546, 132.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.340 - 2.750 |
| R-factor | 0.2447 |
| Rwork | 0.244 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vpt |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17rc1_3605) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.240 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.066 | 0.945 |
| Rmeas | 0.074 | 1.045 |
| Rpim | 0.032 | 0.438 |
| Number of reflections | 7294 | 947 |
| <I/σ(I)> | 14.1 | 1.9 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 5.4 | 5.5 |
| CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 10 MM MNCL2, 10 MM MGCL2, 0.5% PVP K15 |
