6V4Q
Crystal structure of a MR78-like antibody naive-1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.980, 73.120, 105.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 1.390 |
| R-factor | 0.1658 |
| Rwork | 0.165 |
| R-free | 0.18720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w72 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.953 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.780 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmeas | 0.058 | 0.400 |
| Rpim | 0.025 | 0.245 |
| Total number of observations | 495046 | |
| Number of reflections | 99126 | 4511 |
| <I/σ(I)> | 16 | 2.4 |
| Completeness [%] | 99.4 | 92 |
| Redundancy | 5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1% w/v PEG 8000, 20% v/v 2-Propanol, 0.1 M Tris, pH 7.5 |
