6V4C

Culex quinquefasciatus D7 long form 1- CxD7L1 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	I 21 21 21
Unit cell lengths	76.660, 84.320, 132.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.020 - 1.970
R-factor	0.2151
Rwork	0.214
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nht
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.020	39.020	2.020
High resolution limit [Å]	1.970	8.810	1.970
Rmerge	0.066	0.038	0.643
Rmeas	0.072	0.042	0.705
Total number of observations	179520
Number of reflections	30364	332	2235
<I/σ(I)>	12.19	19.71	2.35
Completeness [%]	99.1	83.6	100
Redundancy	5.912	5	5.948
CC(1/2)	0.998	0.998	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES monohydrate, pH 6.5, 25% 550 PEG MME, 0.01 M Zn sulfate