6V0T
Crystal Structure of Catalytic Subunit of Bovine Pyruvate Dehydrogenase Phosphatase 1 - Catalytic Domain
Replaces: 3N3CExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.339, 75.339, 173.255 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.117 - 2.100 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.24660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6q |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.117 | 2.155 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 33626 | 2014 |
<I/σ(I)> | 35.3 | |
Completeness [%] | 96.0 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 298 | The protein solution was 20mg/mL in 10 mM Tris-HCl, pH 7.3, 50 mM NaCl, 1mM MnSO4, 2mM DTT, and 1mM benzamide. The well solution (mixed 1:1) contained 0.10M HEPES, pH 7.5 and 20% Jeffamine M-600., Hanging drop, temperature 298K |