6UZ6

Crystal structure of GLUN1/GLUN2A-4M mutant ligand-binding domain in complex with glycine and glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92013
Spacegroup name	P 21 21 21
Unit cell lengths	54.635, 90.063, 125.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.070 - 1.660
R-factor	0.1811
Rwork	0.179
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nf8
RMSD bond length	0.009
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.100	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.073
Number of reflections	71406	5481
<I/σ(I)>	14.3
Completeness [%]	96.4
Redundancy	6.3	3.9
CC(1/2)		0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM HEPES, pH 7.0, 75 mM sodium chloride, 18% PEG2000 MME