6UYX

Crystal structure of K37-acetylated SUMO1 in complex with phosphorylated DAXX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97750
Spacegroup name	P 1 21 1
Unit cell lengths	34.292, 81.452, 34.284
Unit cell angles	90.00, 112.41, 90.00

Refinement procedure

Resolution	31.690 - 1.700
R-factor	0.169
Rwork	0.159
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wjn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.694	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	18373	1554
<I/σ(I)>	8.6
Completeness [%]	95.8
Redundancy	3.3
CC(1/2)	0.996	0.269

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100MM SODIUM CACODYLATE PH6.5, 26% PEG3350, 10MM CALCIUM CHLORIDE