6UYF

Structure of Hepatitis C Virus Envelope Glycoprotein E2mc3-v1 redesigned core from genotype 6a bound to broadly neutralizing antibody AR3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	175.214, 54.040, 71.486
Unit cell angles	90.00, 94.74, 90.00

Refinement procedure

Resolution	43.650 - 2.060
R-factor	0.1907
Rwork	0.189
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bkc
RMSD bond length	0.004
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.060	2.060
Number of reflections	38846	1408
<I/σ(I)>	15.9
Completeness [%]	93.9
Redundancy	4.6
CC(1/2)	0.960	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG 8000, 0.1M HEPES pH=7.5