6UY4
Crystal structure of dihydroorotate dehydrogenase from Schistosoma mansoni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.980 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 187.981, 187.981, 187.981 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.995 - 2.796 |
R-factor | 0.2174 |
Rwork | 0.216 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6UOY |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.950 |
High resolution limit [Å] | 2.796 | 2.800 |
Number of reflections | 14354 | 2047 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 99.8 | |
Redundancy | 39.8 | |
CC(1/2) | 0.997 | 0.404 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 MES pH 6.5; 1.4 M CITRIC ACID |