6UXH

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-03-15
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 2 21 21
Unit cell lengths	55.640, 126.820, 128.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.070 - 1.858
R-factor	0.2005
Rwork	0.199
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cj0
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.370	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.101	2.124
Rmeas	0.106	2.306
Rpim	0.030	0.888
Total number of observations	957925	29349
Number of reflections	78106	4367
<I/σ(I)>	19.2	0.8
Completeness [%]	99.6	99.7
Redundancy	12.3	6.7
CC(1/2)	0.999	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.175 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 19.5% (w/v) PEG MME 2000