6UWA
Mouse PKC C1B and C2 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2012-02-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.94967 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.041, 58.974, 104.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.390 - 1.200 |
R-factor | 0.1348 |
Rwork | 0.134 |
R-free | 0.15340 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_3126) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.203 | 1.214 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.133 | 0.718 |
Rpim | 0.054 | 0.348 |
Number of reflections | 126177 | 3798 |
<I/σ(I)> | 12 | |
Completeness [%] | 98.2 | |
Redundancy | 5.3 | |
CC(1/2) | 0.996 | 0.658 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 14% PEG 3350, 0.3 mM Lithium sulfate, 50 mM Ammonium citrate tribasic, 0.1 M HEPES pH 7.0 |