6UVO
Structure of antibody 3G12 bound to the central conserved domain of RSV G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.11503 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.330, 139.330, 94.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.530 - 2.900 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.20880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k59 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.067 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.531 | 74.530 | 3.080 |
High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
Rmerge | 0.109 | 0.037 | 0.732 |
Rmeas | 0.126 | 0.043 | 0.847 |
Rpim | 0.062 | 0.022 | 0.419 |
Total number of observations | 3561 | 14475 | |
Number of reflections | 23682 | 934 | 3763 |
<I/σ(I)> | 9.4 | 21.1 | 1.9 |
Completeness [%] | 99.5 | 98.4 | 99.5 |
Redundancy | 3.9 | 3.8 | 3.8 |
CC(1/2) | 0.993 | 0.997 | 0.601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.4 | 277.15 | 1.8M Ammonium Sulfate, 100mM sodium acetate trihydrate pH4.4 |