6UV8
Crystal structure of a far-red cyanobacteriochrome photoreceptor at room temperature
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 297 |
| Detector technology | CCD |
| Collection date | 2018-12-12 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.98-1.2 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 108.800, 108.800, 67.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.657 - 2.701 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4glq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.256 |
| Data reduction software | Precognition |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.038 | 0.746 |
| Number of reflections | 8453 | 397 |
| <I/σ(I)> | 43.4 | 1.3 |
| Completeness [%] | 98.7 | 99.8 |
| Redundancy | 10.3 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.5 | 297 | 2 mg/mL protein, 8.5-10.5% polyethylene glycol 10,000 (PEG), 50 mM NH4CH3COO, and 50 mM Bis-tris buffer at pH 5.5 |
