6UV2

Crystal structure of the core domain of RNA helicase DDX17 with RNA pri-125a-oligo1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97937
Spacegroup name	P 31 2 1
Unit cell lengths	112.722, 112.722, 99.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.810 - 1.894
R-factor	0.1704
Rwork	0.170
R-free	0.19080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DDX17-rU10
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.890	1.890
Rmeas	0.100	0.487
Number of reflections	58330	2922
<I/σ(I)>	55.6
Completeness [%]	99.9
Redundancy	19.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M HEPES pH 7.5, 40% PEG200