6UV1
Crystal structure of RNA helicase DDX17 in complex of rU10 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.752, 91.074, 103.496 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 46.768 - 2.307 |
| R-factor | 0.1995 |
| Rwork | 0.197 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DDX17-rU10 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.175 | 0.832 |
| Number of reflections | 49393 | 2465 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M sodium citrate pH 6.0, 2M NaCl |
