6UUE

The Se-Met Structure of the Vibrio vulnificus ToxR periplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	39.960, 40.530, 49.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.780 - 1.389
R-factor	0.2036
Rwork	0.201
R-free	0.22870
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.780	1.439
High resolution limit [Å]	1.389	1.389
Rmerge	0.063	1.182
Rmeas	0.066	1.258
Rpim	0.018	0.418
Number of reflections	16768	1512
<I/σ(I)>	21.66	1.2
Completeness [%]	98.7
Redundancy	12.1
CC(1/2)	0.999	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	1.7 M Ammonium Sulfate, 0.1 M HEPES pH 7.5, 0.1 M Ammonium formate