6UU8
E. coli mutant sigma-S transcription initiation complex with a 7-nt RNA ("Fresh" mutant crystal soaked with GTP, UTP, and CTP for 30 minutes)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.460, 154.504, 235.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.378 - 4.400 |
| Rwork | 0.357 |
| R-free | 0.37960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.602 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 4.660 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Number of reflections | 31470 | 4856 |
| <I/σ(I)> | 6.92 | 0.44 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 6 | 5.9 |
| CC(1/2) | 0.998 | 0.178 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG3350, sodium chloride, HEPES |
