6UU5

E. coli sigma-S transcription initiation complex with a 6-nt RNA ("Old" crystal soaked with GTP, UTP, CTP, and dinucleotide GpA for 30 minutes)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	132.358, 153.766, 231.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 5.403
Rwork	0.295
R-free	0.36250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ipl
RMSD bond length	0.005
RMSD bond angle	1.347
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	5.730
High resolution limit [Å]	5.400	5.400
Number of reflections	16704	2577
<I/σ(I)>	6.68	0.55
Completeness [%]	99.4	97.8
Redundancy	6.2	5
CC(1/2)	0.996	0.133

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	PEG3350, sodium chloride, HEPES