6UU5
E. coli sigma-S transcription initiation complex with a 6-nt RNA ("Old" crystal soaked with GTP, UTP, CTP, and dinucleotide GpA for 30 minutes)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 132.358, 153.766, 231.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 5.403 |
Rwork | 0.295 |
R-free | 0.36250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ipl |
RMSD bond length | 0.005 |
RMSD bond angle | 1.347 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 5.730 |
High resolution limit [Å] | 5.400 | 5.400 |
Number of reflections | 16704 | 2577 |
<I/σ(I)> | 6.68 | 0.55 |
Completeness [%] | 99.4 | 97.8 |
Redundancy | 6.2 | 5 |
CC(1/2) | 0.996 | 0.133 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG3350, sodium chloride, HEPES |