6UTU

Crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa in the P3 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	1.0332
Spacegroup name	P 3
Unit cell lengths	158.100, 158.100, 64.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.910 - 2.850
R-factor	0.2183
Rwork	0.214
R-free	0.29410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vtm
RMSD bond length	0.009
RMSD bond angle	1.102
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	3.000
High resolution limit [Å]	2.830	2.830
Number of reflections	42465	6545
<I/σ(I)>	12.77
Completeness [%]	99.2
Redundancy	10
CC(1/2)	0.998	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	19 %-22 % PEG 2000 MME, 0.1 M Tris, pH 8.0-9.0, 0.2 M trimethylamine N-oxide (TMAO)