6UTN

Native E. coli Glyceraldehyde 3-phosphate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-02
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	77.673, 187.353, 121.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.840 - 1.790
R-factor	0.1782
Rwork	0.178
R-free	0.19860
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.324
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.840	1.820
High resolution limit [Å]	1.790	1.790
Number of reflections	746389	83268
<I/σ(I)>	37.7
Completeness [%]	99.8
Redundancy	9
CC(1/2)	0.870	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	5.5 mg/mL protein, 0.1 M HEPES pH 7.5 and 1.43 M sodium citrate