6UTK

Crystal structure of 438-B11 Fab in complex with an uncleaved prefusion optimized (UFO) soluble BG505 trimer and Fab 35O22 at 3.80 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	H 3 2
Unit cell lengths	247.308, 247.308, 257.612
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.440 - 3.800
R-factor	0.2999
Rwork	0.299
R-free	0.31730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
RMSD bond length	0.004
RMSD bond angle	0.774
Data reduction software	HKL-2000 (v0.95)
Data scaling software	SCALEPACK (1.16_3549)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.870
High resolution limit [Å]	3.800	3.800
Rpim	0.050	0.420
Number of reflections	29804	1403
<I/σ(I)>	13
Completeness [%]	99.6
Redundancy	9.9
CC(1/2)	0.890	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M sodium chloride, 36% v/v PEG400, 0.1 M sodium/potassium phosphate, pH 5.7