6USQ

Telomerase Reverse Transcriptase binary complex with Y256A mutation, TERT:DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-10
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 61 2 2
Unit cell lengths	101.009, 101.009, 379.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.900 - 3.620
R-factor	0.282
Rwork	0.281
R-free	0.29670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kyl
RMSD bond length	0.006
RMSD bond angle	1.149
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	3.670
High resolution limit [Å]	3.400	3.400
Number of reflections	16740	3311
<I/σ(I)>	9
Completeness [%]	99.7
Redundancy	18.3
CC(1/2)	0.999	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	11% isopropanol, 0.1 M KCl, 25 mM MgCl2, and 50 mM sodium cacodylate