6USM
Structure of nuclease domain of human parvovirus B19 non-structural protein 1 in complex with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2013-09-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.690, 102.690, 134.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 3.370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MBP |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.955 | 48.000 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Rmerge | 0.125 | |
| Number of reflections | 11564 | 11564 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 96.8 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.2 M LiSo4 and sodium acetate tri-hydrate buffer of pH4.6 |
