6USC
Structure of Human Intelectin-1 in complex with KO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 118.145, 118.145, 118.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.230 - 1.590 |
| R-factor | 0.1594 |
| Rwork | 0.158 |
| R-free | 0.18220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wmq |
| Data reduction software | DIALS (1.9.2) |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.240 | 1.630 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.140 | 0.893 |
| Rmeas | 0.142 | 0.938 |
| Rpim | 0.023 | 0.273 |
| Number of reflections | 73205 | 4888 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.2 | 91.2 |
| Redundancy | 35.8 | 10.3 |
| CC(1/2) | 0.999 | 0.488 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM bis-Tris, 25% PEG 3350 |
