6UR5

Resurfaced influenza hemagglutinin in complex with a broadly neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999
Spacegroup name	C 1 2 1
Unit cell lengths	92.270, 141.470, 166.870
Unit cell angles	90.00, 102.55, 90.00

Refinement procedure

Resolution	48.590 - 4.000
R-factor	0.301
Rwork	0.300
R-free	0.32180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ha0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.590	48.590	4.200
High resolution limit [Å]	4.000	46.000	4.000
Rmerge	0.290	0.041	0.734
Rmeas	0.340	0.048	0.875
Total number of observations	63077
Number of reflections	17321	2	2162
<I/σ(I)>	4.51	21.74	1.67
Completeness [%]	97.5	14.3	89.6
Redundancy	3.642	4	3.284
CC(1/2)	0.950		0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM sodium citrate (pH 4.5), 20% (wt/vol) PEG 4000