6UR2

DNA polymerase I Large Fragment from Bacillus stearothermophilus with DNA template and primer containing an N3'-> P5' linkage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2018-12-14
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	85.197, 93.679, 107.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.850 - 2.270
R-factor	0.2313
Rwork	0.229
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l5u
RMSD bond length	0.008
RMSD bond angle	1.550
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.270	2.280
Rmerge	0.120	0.508
Rmeas	0.129	0.543
Rpim	0.046	0.192
Number of reflections	39951	3928
<I/σ(I)>	17.9	4.7
Completeness [%]	99.9	100
Redundancy	8	8
CC(1/2)	0.991	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	10mM MgCl2, 0.8 M Succinic acid pH 7.0