6UQ2
RNA polymerase II elongation complex with dG in state 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.688, 223.317, 193.516 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 49.754 - 3.200 |
| R-factor | 0.2305 |
| Rwork | 0.230 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6blo |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.754 | 3.140 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.333 | 4.636 |
| Rmeas | 0.357 | 4.969 |
| Rpim | 0.128 | 1.784 |
| Total number of observations | 48106 | |
| Number of reflections | 126625 | 6215 |
| <I/σ(I)> | 7.6 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.7 |
| CC(1/2) | 0.992 | 0.270 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
